TY - JOUR AU - Tangmahakul, Nattawan AU - Techangamsuwan, Somporn AU - Disatian Surachetpong, Sirilak PY - 2020/01/07 Y2 - 2024/03/29 TI - Selection of the appropriate reference genes for relative quantitative reverse transcription polymerase chain reaction (qRT-PCR) in canine pulmonary arteries JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 49 IS - 4 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/234302 SP - 313-318 AB - <p><span class="fontstyle0">A normalisation of targeted genes using the appropriate reference genes is necessary for reliable quantitative<br>reverse transcription polymerase chain reaction (qRT-PCR) experiments. This study aimed to investigate the<br>appropriate reference genes and the numbers of the genes required for qRT-PCR in canine pulmonary arteries.<br>Pulmonary arteries were collected from twenty-three dogs at necropsy. Candidate reference genes including ribosomal<br>protein L32 (RPL32), ribosomal protein L13a (RPL13A), ribosomal protein S18 (RPS18), TATA box binding protein<br>(TBP), beta-2-microglobulin (B2M), ribosomal protein S5 (RPS5), ribosomal protein S19 (RPS19) and glyceraldehyde-3-<br>phosphate dehydrogenase (GAPDH) from pulmonary arteries were examined by qRT-PCR and analysed using four<br>major algorithms, including geNorm, NormFinder, the comparative delta-C</span><span class="fontstyle0">T </span><span class="fontstyle0">method, and RefFinder. The optimal<br>number of genes was analysed using geNorm. The ranking of the appropriate reference genes from all algorithms was<br>evaluated by RefFinder. The appropriate reference genes for the normalisation of the targeted gene in canine<br>pulmonary arteries are RPS19 and RPL32.</span> </p> ER -