TY - JOUR AU - Macotpet, Arayaporn AU - Pattarapanwichien, Ekkachai AU - Suksawat, Fanan AU - Boonsiri, Patcharee PY - 2019/03/25 Y2 - 2024/03/29 TI - Oxidative stress and antioxidant in canine cutaneous mast cell tumors JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 48 IS - 4 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/179841 SP - 631-637 AB - <p><span class="fontstyle0">Oxidative stress can result from either the excessive production of reactive oxygen species (ROS) or an<br>impaired antioxidant system, or both. It causes damage to lipids, proteins and DNA. Therefore, oxidative stress may<br>be involved in carcinogenesis, and is associated with many types of cancer in dogs. The objective of this study was to<br>compare the levels of malondialdehyde, protein hydroperoxides, glutathione, retinol and alpha-tocopherol between<br>dogs with mast cell tumors and clinically healthy controls. Blood samples were obtained from eighteen clinically<br>healthy dogs and fourteen dogs with spontaneous mast cell tumors. Malondialdehyde and protein hydroperoxides<br>levels were measured by the thiobarbituric acid reactive substance assay, and the ferric-xylenol orange assay,<br>respectively. Glutathione level was determined using spectrophotometric assay. Retinol and alpha-tocopherol levels<br>were measured using the high performance liquid chromatographic method. Dogs with mast cell tumors had<br>significantly higher levels of malondialdehyde (P&lt;0.01) and protein hydroperoxides (P&lt;0.05) compared with the<br>clinically healthy controls. When considering antioxidants, dogs with mast cell tumors had significantly lower levels of<br>glutathione (P&lt;0.01), retinol (P&lt;0.05) and alpha-tocopherol (P&lt;0.01) compared with the clinically healthy controls. Mast<br>cell tumors in dogs are associated with oxidative stress and antioxidant status. Further studies on oxidative stress and<br>antioxidant activity in dogs should be conducted to guide and plan the complementary treatment of canine cancer.</span> </p> ER -