TY - JOUR AU - Tawatsin, Apiwat AU - Phumee, Atchara AU - Thavara, Usavadee AU - Sirisopa, Patcharawan AU - Ritthison, Wanapa AU - Thammakosol, Kitsanaphong AU - Intayot, Proawpilart AU - Joyjinda, Yutthana AU - Wacharapluesadee, Supaporn AU - Hemachudha, Thiravat AU - Siriyasatien, Padet PY - 2019/03/11 Y2 - 2024/03/29 TI - High infection rate of Zika virus in mosquitoes collected from an area of active Zika virus transmission in eastern Thailand JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 48 IS - 4 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/176923 SP - 551-558 AB - <p><span class="fontstyle0">Zika virus (ZIKV), chikungunya virus (CHIKV) and dengue virus (DENV) are emerging and re-emerging<br>arboviral diseases. These viruses are transmitted to humans through the bites of </span><span class="fontstyle2">Aedes </span><span class="fontstyle0">mosquitoes. Recently, ZIKV<br>infection has been described as an emerging disease in Thailand and many countries, especially in tropical and subtropical areas. Specific drugs and vaccines against these infections are unavailable; therefore, effective disease control<br>relies on vector control measures only. To understand the transmission cycle of these viruses and mosquito vectors,<br>this study is designed to investigate the natural infection of ZIKV, CHIKV and DENV in field-caught mosquitoes by<br>molecular techniques. Adults and larvae of mosquitoes were collected in and around the patients’ homes in the Klaeng<br>District, Rayong Province, Thailand. CHIKV and DENV were detected by Multiplex Real-time RT-PCR and ZIKV was<br>detected by Hemi-nested RT-PCR. ZIKV RNA was detected in 8 (10.3%) samples (5 (6.4%) females and 2 (2.6%) males<br>of </span><span class="fontstyle2">Aedes aegypti </span><span class="fontstyle0">and 1 (1.3%) female </span><span class="fontstyle2">Armigeres subalbatus</span><span class="fontstyle0">) and CHIKV RNA in 5 (6.4%) (3 (3.8%) females and 2 (2.6%)<br>larvae of </span><span class="fontstyle2">Ae. aegypti</span><span class="fontstyle0">), while DENV RNA was not detected in any samples. The Maximum Likelihood tree of nucleotide<br>sequences of positive samples showed that ZIKV in mosquitoes were cladded within the Asian lineage. This study was<br>a preliminary survey of the potential vectors of ZIKV, CHIKV and DENV in an affected area. Information obtained<br>from this study helps to understand the natural infection rates in mosquitoes with ZIKV, CHIKV and DENV and may<br>be valuable in creating the most effective mosquito vector control strategies in the future.</span> </p> ER -