TY - JOUR AU - Surachetpong, Win AU - Nantakhruea, Sunaree AU - Inthasaeng, Phiangfhun AU - Lekcharoensuk, Porntippa PY - 2018/08/03 Y2 - 2024/03/29 TI - ERK MAPK signaling pathway regulates porcine circovirus type 2 replication in peripheral blood mononuclear cells JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 48 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/138028 SP - 171-178 AB - <p><span class="fontstyle0">Porcine circovirus type 2 </span><span class="fontstyle2">(</span><span class="fontstyle0">PCV2</span><span class="fontstyle2">) </span><span class="fontstyle0">is one of the most important swine viral diseases in Thailand and worldwide</span><span class="fontstyle2">.<br></span><span class="fontstyle0">While the interaction between PCV2 and pig immune system has been largely explored, the mechanisms by which<br>PCV2 replicates in porcine immune cells and alters host cellular signaling pathways remain incomplete</span><span class="fontstyle2">. </span><span class="fontstyle0">In this study,<br>extracellular regulated kinase </span><span class="fontstyle2">(</span><span class="fontstyle0">ERK</span><span class="fontstyle2">) </span><span class="fontstyle0">phosphorylation form was shown to slightly increase in PCV2</span><span class="fontstyle2">-</span><span class="fontstyle0">infected peripheral<br>blood mononuclear cells </span><span class="fontstyle2">(</span><span class="fontstyle0">PBMCs</span><span class="fontstyle2">) </span><span class="fontstyle0">at 48 hr post infection</span><span class="fontstyle2">. </span><span class="fontstyle0">To demonstrate the role of ERK MAPK signaling, PBMCs<br>were divided into three groups including control, virus, and virus with the mitogen</span><span class="fontstyle2">-</span><span class="fontstyle0">activated protein kinase </span><span class="fontstyle2">(</span><span class="fontstyle0">MEK</span><span class="fontstyle2">)<br></span><span class="fontstyle0">specific inhibitor, U0126, to prevent ERK phosphorylation</span><span class="fontstyle2">. </span><span class="fontstyle0">U0126 at 10 </span><span class="fontstyle3">ยต</span><span class="fontstyle0">M completely inhibited ERK activation in the<br>porcine PBMCs at 48 and 72 hr</span><span class="fontstyle2">. </span><span class="fontstyle0">Interestingly, the inhibition of ERK MAPK significantly impaired PCV2 replication in<br>the PBMCs</span><span class="fontstyle2">. </span><span class="fontstyle0">In summary, our results unraveled the role of ERK MAPK signaling during PCV2 replication in porcine<br>immune cells</span><span class="fontstyle2">. </span><span class="fontstyle0">These findings provide important information on how PCV2 interacts with host cells and influences host<br>immune response</span><span class="fontstyle2">.</span> </p> ER -