@article{Thiangthientham_Anakkul_Suwimonteerabutr_Kallayanathum_Santiviparat_Techakumphu_Tharasanit_2022, title={Ovarian stimulation with a low dose of follicle stimulating hormone improved recovery rates and developmental competence of goat oocytes}, volume={52}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/258551}, abstractNote={<p>The aim of this study was to optimise the ovarian responses using different FSH (follicle stimulating hormone) treatments in goats. Group 1 (control), the ovaries were stimulated with a total dose of 200 mg FSH per doe. The FSH injections were performed at 12-h intervals starting on day 9 after progesterone implantation. The FSH doses were decreased to 160 mg/doe and 130 mg/doe for Groups 2 and 3, respectively. The numbers of small, medium and large follicles were determined by real-time B mode ultrasonography. Ovum pick-up (OPU) was performed by laparoscopy under general anesthesia at 96, 42 and 36 h after the initial dose of FSH for Group 1, 2 and 3, respectively. The numbers of recovered cumulus-oocyte-complexes (COCs) and their quality were examined. The COCs were further matured and fertilised in vitro. It was found that the numbers of small- and medium-sizes follicles in Group 2 and 3 were significantly higher than those in Group 1, whereas the numbers of large follicles in Group 1 were significantly higher than those in the other groups (p < 0.05). There was no significant difference in oocyte recovery rates and the quality of COCs among groups (p > 0.05). However, the low-dose FSH (Group 3) tended to enhance oocyte recovery rates and oocyte quality compared to Group 1 (p = 0.07 and 0.06, respectively). Although the meiotic competence of recovered oocytes from Group 3 was similar to that of Group 1, cleavage rates were significantly greater than those in Group 1 (52.30 vs. 30.15%, respectively). In conclusion, ovarian stimulation with a low dose of FSH, related to the interval time between the first FSH injection and the OPU, improved oocyte recovery rates and the developmental competence of goat oocytes.</p>}, number={3}, journal={The Thai Journal of Veterinary Medicine}, author={Thiangthientham, Pintira and Anakkul, Nitira and Suwimonteerabutr, Junpen and Kallayanathum, Wirakan and Santiviparat, Sawita and Techakumphu, Mongkol and Tharasanit, Theerawat}, year={2022}, month={Sep.}, pages={507–513} }