@article{Kemthong_Sapkanarak_Rounmakok_Sing-ayudthaya_Suriya-arunroj_Föger_Werle_Malaivijitnond_2021, title={A rapid fecal immunochemical test for gastrointestinal bleeding detection in cynomolgus macaques (Macaca fascicularis)}, volume={51}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/248718}, abstractNote={<p><span class="fontstyle0">We used the Fecal Immunochemical Occult Blood Test </span><span class="fontstyle2">(</span><span class="fontstyle0">FIT</span><span class="fontstyle2">) </span><span class="fontstyle0">kit, a lateral flow immunochromatographic assay specific for human hemoglobin, on cynomolgus monkeys </span><span class="fontstyle2">(</span><span class="fontstyle3">Macaca fascicularis</span><span class="fontstyle2">). </span><span class="fontstyle0">We aimed to employ this test to detect gastrointestinal injury in captive animals at the National Primate Research Center of Thailand</span><span class="fontstyle2">-</span><span class="fontstyle0">Chulalongkorn University </span><span class="fontstyle2">(</span><span class="fontstyle0">NPRCT</span><span class="fontstyle2">-</span><span class="fontstyle0">CU</span><span class="fontstyle2">). </span><span class="fontstyle0">The cross</span><span class="fontstyle2">-</span><span class="fontstyle0">reactivity and sensitivity of the test for monkey blood were determined and compared with the human blood</span><span class="fontstyle2">. </span><span class="fontstyle0">The anti</span><span class="fontstyle2">-</span><span class="fontstyle0">human hemoglobin antibody of the FIT kit reacted with the monkey blood in a similar way as it did with humans and the intensity </span><span class="fontstyle2">(</span><span class="fontstyle0">T</span><span class="fontstyle2">/</span><span class="fontstyle0">C ratio</span><span class="fontstyle2">) </span><span class="fontstyle0">values between the two data sets closely correlated </span><span class="fontstyle2">(</span><span class="fontstyle0">R</span><span class="fontstyle0">2 </span><span class="fontstyle2">= </span><span class="fontstyle0">0</span><span class="fontstyle2">.</span><span class="fontstyle0">9324, </span><span class="fontstyle3">p</span><span class="fontstyle0">>0</span><span class="fontstyle2">.</span><span class="fontstyle0">05</span><span class="fontstyle2">). </span><span class="fontstyle0">Although the specificity for monkey blood was 4</span><span class="fontstyle2">.</span><span class="fontstyle0">2 times lower than for human blood, monkey blood with a dilution as low as 1</span><span class="fontstyle2">:</span><span class="fontstyle0">256,000 could be detected in monkey fecal samples</span><span class="fontstyle2">. </span><span class="fontstyle0">Thus, we used the FIT kit to determine the gastrointestinal adverse effects of the NPRCT excipient which was orally administered daily to four female cynomolgus monkeys for 28 days. A daily visual inspection of freshly defecated fecal samples did not detect any blood but with the high sensitivity of the FIT kit the presence of hemoglobin in the feces was shown once in three of the four monkeys (3 of the 112 specimens determined). In conclusion, the human</span><span class="fontstyle2">-</span><span class="fontstyle0">FIT can be an applicable tool for early detection of gastrointestinal injury in captive as well as wild cynomolgus monkeys</span><span class="fontstyle2">. </span><span class="fontstyle0">The advantage of this kit is that it requires non</span><span class="fontstyle2">-</span><span class="fontstyle0">invasive fecal sample collection, requires no additional equipment and gives results that can be read by the naked eye immediately</span><span class="fontstyle2">.</span> </p>}, number={2}, journal={The Thai Journal of Veterinary Medicine}, author={Kemthong, Taratorn and Sapkanarak, Krittiga and Rounmakok, Piyachada and Sing-ayudthaya, Thipchompoo and Suriya-arunroj, Lalitta and Föger, Florian and Werle, Martin and Malaivijitnond, Suchinda}, year={2021}, month={Mar.}, pages={331–337} }