@article{Zhou_Gao_Zheng_Wu_Cao_Zhang_Cong_2021, title={Molecular evolutionary analysis of ORF3 and M genes of porcine epidemic diarrhea virus in HeiLongJiang province of China}, volume={51}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/247459}, abstractNote={<p><span class="fontstyle0">In this present study, 11 porcine epidemic diarrhea viruses (PEDVs) were isolated from the fecal samples of piglets<br>infected with PEDV in HeiLongJiang province of China. In order to analyze the molecular evolution of these reemerging PEDVs, the ORF3 and M genes of 11 PEDVs were amplified by reverse transcriptase polymerase chain<br>reaction, cloned, sequenced, and analyzed with each other as well as other PEDV reference strains. The complete<br>nucleotide sequence comparison was performed with Bioedit software, and the results showed that the similarity of 11<br>ORF3 and M genes ranged from 97% to 100%. Nucleotides and amino acids were changed at some sites in the ORF3<br>and M genes of the 11 PEDV isolates when compared with CV777 reference strain, indicating that high level of variation<br>may have occurred in the ORF3 and M genes of PEDV strains. To establish genetic relationships of the fully sequenced<br>ORF3 and M genes, multiple sequence alignments were carried and phylogenetic analysis was performed. The results<br>indicated that these PEDV isolates belonged to genotype 3.4 that is dominant subgroup in PEDV Group, showed a close<br>relationship with some recent reported Chinese PEDV strains, and exhibited comparatively rapid genetic evolution, as<br>revealed by these viruses responsible for the recent PEDV outbreak in HeiLongJiang province clustered closely together<br>with other Chinese strains with rapid evolution rate. This study might be not only crucial to understand the currently<br>prevailing PEDV strains in northeast of China, but also provides valuable data for preventing and controlling PEDV.</span> </p>}, number={1}, journal={The Thai Journal of Veterinary Medicine}, author={Zhou, Yulong and Gao, Guoqiang and Zheng, Liang and Wu, Zhijun and Cao, Hongwei and Zhang, Hua and Cong, Yanlong}, year={2021}, month={Jan.}, pages={185–195} }