@article{Prasertsincharoen_Metheenukul_2020, title={Production of immunoglobulin Y against dog erythrocyte antigen (DEA) 1.1 from egg yolk}, volume={50}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/245848}, abstractNote={<p><span class="fontstyle0">The dog erythrocyte antigen (DEA) as a blood group antigen in dogs, especially DEA 1.1, has the highest<br>immunogenicity. IgY is an excellent antibody for use in immunological assays involving mammalian sera. The aim of<br>this study was to produce and purify IgY against DEA1.1 from chicken egg yolks for canine blood group detection.<br>Hens were immunized by intramuscular vaccination. IgY was achieved after three booster immunizations by DEA 1.1-<br>positive canine ghost cells. The extraction of total IgY from the egg yolk was carried out using precipitation by<br>polyethylene glycol 6000 and dialysis. The purified protein and yield of IgY were 102.52 ± 0.13 mg/ml and 25.13 ±<br>2.45%. Electrophoretic separation of egg yolk IgY showed other bands that appeared between the heavy and light<br>chains of IgY corresponding to molecular weights between 65 and 27</span><span class="fontstyle2"> </span><span class="fontstyle0">kDa. Canine red blood cells with DEA1.1 positive<br>which agglutinated with monoclonal antibody against DEA1.1 in a Rapid Vet H detection kit reacted with polyclonal<br>antibody against DEA1.1. Hemagglutination of three sample-polyclonal antibodies against DEA1.1 and two samples<br>of canine red blood cell with DEA1.1 positive was shown at titer 4. The purified IgY can be standardized and relatively<br>simple to perform, compared with standardization for blood typing. We successfully purified chicken IgY against<br>DEA1.1 from egg yolks of immunized hens, which can be used for diagnostic reagent for canine blood group<br>DEA1.1 detection. It is expected that IgY will play an increasing role in research and diagnosis in the future.</span> </p>}, number={3}, journal={The Thai Journal of Veterinary Medicine}, author={Prasertsincharoen, Noppadol and Metheenukul, Pornphimon}, year={2020}, month={Sep.}, pages={381–387} }