@article{Sananmuang_Singto_Intarak_Sanansin_Budsasom_Kongthip_Wannaratana_2020, title={Optimizing system for sperm quality evaluation using crystalloid diluent for pigeons (Columba livia domestica)}, volume={50}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/244075}, abstractNote={<p><span class="fontstyle0">Semen quality is a crucial factor for predicting fertility and hatchability in several avian species including pigeons.<br>To evaluate pigeon semen quality, prior dilution of semen was required due to its small volume with highly<br>concentrated spermatozoa. Conventional crystalloid solutions—Normal saline (NSS) and Ringer’s solution (LRS) are<br>common diluents for such a task; however, the unoptimized dilution rate of such diluents was a major cause<br>contributing to inaccurate semen quality evaluation. Due to the lack of such knowledge in pigeons this study aimed to<br>optimize the dilution rate of Normal saline (NSS) and Ringer’s solution (LRS) for pigeon semen quality evaluation. The<br>semen of a total of 20 male racing pigeons was collected and diluted in NSS or LRS at 12.5-, 25-, 50-, 100- and 200-fold<br>dilutions. Immediate percentages of sperm motility, progressive motility and viability were determined for each fold<br>dilution. The 12.5- and 25-fold-diluted semen were further evaluated at 0, 0.5, 1 and 3 h of storage. The results showed<br>that NSS and LRS rendered indifferent effects on immediate semen quality. However fold dilutions higher than 25<br>resulted in a great decline in semen quality both in NSS and LRS. Storage of 12.5-fold and 25-fold diluted semen resulted<br>in a marked decrease of semen quality as soon as 0.5 h; however, LRS was better than NSS in terms of maintaining<br>semen quality. This study thus supports immediate quality evaluation of pigeon semen—by which 12.5-25 folddilutions were suggested.</span> </p>}, number={2}, journal={The Thai Journal of Veterinary Medicine}, author={Sananmuang, Thanida and Singto, Thitirat and Intarak, Kanyavee and Sanansin, Pimnipa and Budsasom, Muttana and Kongthip, Arrita and Wannaratana, Suwarak}, year={2020}, month={Jun.}, pages={203–209} }