@article{Thiangthientham_Suwimonteerabutr_Tharasanit_Techakumphu_2020, title={The optimal divalent cations and storage temperatures for the encapsulation of ram spermatozoa}, volume={50}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/243261}, abstractNote={<p><span class="fontstyle0">The aim of this study was to investigate the effects of encapsulation with different divalent cations and temperatures<br>on the quality of ram spermatozoa during cold storage. Experiment 1: diluted semen was allocated randomly into three<br>groups: control, encapsulation with calcium (Ca</span><span class="fontstyle0">2+</span><span class="fontstyle0">) alginate and encapsulation with barium (Ba</span><span class="fontstyle0">2+</span><span class="fontstyle0">) alginate. The samples<br>were stored at two different temperatures (4 °C or 16 °C) for 24 h and subsequently examined for spermatozoa quality.<br>Experiment 2: determination of sperm functionality by means of sperm binding was performed. Spermatozoa with and<br>without Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate were cooled for 24 h and then used for sperm binding assay. Experiment 1: the progressive<br>motility of spermatozoa in Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate stored at 16 °C was better than that stored at 4 °C (P = 0.042). Encapsulation<br>with Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate and storage at 16 °C significantly improved progressive motility when compared to Ca</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate<br>(</span><span class="fontstyle2">P </span><span class="fontstyle0">< 0.001). The motility characteristics of curve velocity, straight linear velocity and amplitude lateral head<br>displacement of Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate at 16 °C were higher than Ca</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate (P = 0.045, P = 0.005 and P = 0.013, respectively).<br>A low storage temperature (4 °C and 16 °C) did not markedly decrease the viability or acrosome integrity of<br>spermatozoa, irrespective the type of crosslinking. Although the spermatozoa released from Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate were motile<br>and could bind to the zona pellucida, the numbers of bound spermatozoa were significantly lower than in the control<br>group (</span><span class="fontstyle2">P </span><span class="fontstyle0">< 0.001, Experiment 2). In conclusion, Ba</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate is preferable to Ca</span><span class="fontstyle0">2+ </span><span class="fontstyle0">alginate for the encapsulation of ram<br>spermatozoa with cold storage at 16 °C.</span> </p>}, number={1}, journal={The Thai Journal of Veterinary Medicine}, author={Thiangthientham, Pintira and Suwimonteerabutr, Junpen and Tharasanit, Theerawat and Techakumphu, Mongkol}, year={2020}, month={Jun.}, pages={89–96} }