@article{Aiemsaard_Kamollerd_Uopasai_Singh_Thongkham_2020, title={Efficiency of clove essential oil against planktonic cells and biofilms of Malassezia pachydermatis isolated from canine dermatitis}, volume={49}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/241553}, abstractNote={<p><span class="fontstyle0">This study investigated the ability of clove oil and eugenol to inhibit biofilm production and eradicate canine<br></span><span class="fontstyle2">Malassezia pachydermatis </span><span class="fontstyle0">in biofilm form. Biofilm formation was studied using a crystal violet assay and was quantified<br>by optical density (OD) at 595 nm. Killing time was assessed by time-kill assay. All 17 isolates of canine </span><span class="fontstyle2">M. pachydermatis<br></span><span class="fontstyle0">were capable of biofilm formation, however, 6 isolates formed more extensive biofilms than the other isolates and were<br>selected for susceptibility testing. Clove essential oil and eugenol showed effectiveness against both planktonic and<br>biofilm cells with identical minimum planktonic inhibitory concentration (MPIC</span><span class="fontstyle0">50</span><span class="fontstyle0">) and minimum planktonic fungicidal<br>concentration (MPFC</span><span class="fontstyle0">50</span><span class="fontstyle0">), 0.156 and 0.312 mg/ml, respectively. The minimum biofilm inhibitory concentration (MBIC</span><span class="fontstyle0">50</span><span class="fontstyle0">)<br>and the minimum biofilm eradication concentration (MBEC</span><span class="fontstyle0">50</span><span class="fontstyle0">) were 0.312 and 0.625 mg/ml, respectively. These studies<br>indicate that biofilms of </span><span class="fontstyle2">M. pachydermatis </span><span class="fontstyle0">are more resistant to clove essential oil and eugenol. In addition, eugenol is<br>the component of clove essential oil responsible for its antifungal activity. The results of the time-kill study showed<br>that the antimicrobial activity of clove essential oil was time and concentration-dependent.</span> </p>}, number={4}, journal={The Thai Journal of Veterinary Medicine}, author={Aiemsaard, Jareerat and Kamollerd, Chuchat and Uopasai, Suwit and Singh, Ranee and Thongkham, Eakachai}, year={2020}, month={Apr.}, pages={415–420} }