@article{Lothong_Wattanaphansak_Deachapunya_Poonyachoti_2020, title={Porcine reproductive and respiratory syndrome virus (PRRSV) preferentially infected the apical surface of primary endometrial cell monolayer}, volume={49}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/240500}, abstractNote={<p><span class="fontstyle0">The underlying mechanism of porcine reproductive and respiratory syndrome virus (PRRSV) causing reproductive<br>failure and re-circulation in herds has remained unclear. Endometrial cells primarily infected with PRRSV may serve<br>as a significant target for PRRSV eradication. Primary endometrial (PE) cells from the porcine uterus were isolated and<br>cultivated to pursue this possibility. Immunocytochemistry analysis revealed the protein expression of classical<br>estrogen receptors (ER-</span><span class="fontstyle2"> </span><span class="fontstyle0">and ER-</span><span class="fontstyle2"></span><span class="fontstyle0">), but not PRRSV receptors, CD163 and sialoadhesin in PE cells. PE cells were<br>apically/basolaterally inoculated with PRRSV type I/type II isolated from PRRSV infected lungs or mock infection.<br>Cytopathic effects (CPE) and PRRSV-GP5 positive cells were detected in PE cells incubated with PRRSV inoculum (10</span><span class="fontstyle0">7<br></span><span class="fontstyle0">TCID</span><span class="fontstyle0">50</span><span class="fontstyle0">/ml) beginning at 4 days post inoculation (dpi). Only apical inoculation produced effects, suggesting route<br>dependence of PRRSV infectivity in PE cells (</span><span class="fontstyle3">p<0.05</span><span class="fontstyle0">). PRRSV type II produced overall effects i.e., CPE, PRRSV-GP5<br>positive cells and a viral load higher than type I (</span><span class="fontstyle3">p<0.05</span><span class="fontstyle0">) during 2-6 dpi. In accordance with these effects, the tissue<br>epithelial resistance (TER) of type II inoculated PE cells was lower than that of mock or type I inoculated cells (</span><span class="fontstyle3">p<0.05</span><span class="fontstyle0">).<br>In addition, all the PE cells and media samples collected from PRRSV-inoculated PE cells persistently revealed PRRSVGP5 protein and viral copies (10</span><span class="fontstyle0">2</span><span class="fontstyle0">-10</span><span class="fontstyle0">8 </span><span class="fontstyle0">TCID</span><span class="fontstyle0">50</span><span class="fontstyle0">/ml) accessed by infecting MARC-145 cells. These findings provided the<br>first evidence that PE cells can be directly infected with PRRSV, favorably by type II at the apical side. However, all<br>PRRSV contaminated PE cells persistently carry the progeny virus.</span> </p>}, number={4}, journal={The Thai Journal of Veterinary Medicine}, author={Lothong, Muttarin and Wattanaphansak, Suphot and Deachapunya, Chatsri and Poonyachoti, Sutthasinee}, year={2020}, month={Mar.}, pages={401–413} }