@article{Sangwiman_Phothaworn_Muangsombut_Chanprasert_Chaichana_Tangjittipokin_Korbsrisate_2019, title={Production and characterization of polyclonal antibody against major capsid protein of Salmonella bacteriophage SE-W109}, volume={49}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/223903}, abstractNote={<p><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp</span><span class="fontstyle0">., </span><span class="fontstyle2">are important foodborne pathogens that are predominantly found in poultry, swine, eggs and dairy<br>products</span><span class="fontstyle3">. </span><span class="fontstyle2">Rapid detection of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">contamination in animals and food is urgently needed to reduce the risk of<br>infection</span><span class="fontstyle3">. </span><span class="fontstyle2">Bacteriophages or phages are viruses that specifically infect and kill bacteria. Our research team at the<br>Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand isolated<br></span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phage SE</span><span class="fontstyle3">-</span><span class="fontstyle2">W109 that demonstrated a broad host range of infection to various serovars of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp</span><span class="fontstyle3">.</span><span class="fontstyle2">, but<br>that did not cross</span><span class="fontstyle3">-</span><span class="fontstyle2">react with other Gram</span><span class="fontstyle3">-</span><span class="fontstyle2">negative bacteria</span><span class="fontstyle3">. </span><span class="fontstyle2">We then set forth to develop this phage for the rapid detection<br>of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. contamination in food samples</span><span class="fontstyle3">. </span><span class="fontstyle2">However, to achieve this objective, a polyclonal antibody against<br></span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phage SE</span><span class="fontstyle3">- </span><span class="fontstyle2">W109 is required</span><span class="fontstyle3">. </span><span class="fontstyle2">In the present study, an optimization condition for rabbit immunization to<br>generate an anti</span><span class="fontstyle3">- </span><span class="fontstyle2">phage antibody was successfully developed</span><span class="fontstyle3">. </span><span class="fontstyle2">Western blot analysis revealed that the generated<br>antibody recognized the 45 kDa antigen of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phage SE</span><span class="fontstyle3">-</span><span class="fontstyle2">W109 and that it had no cross</span><span class="fontstyle3">-</span><span class="fontstyle2">reaction with other<br></span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phages or with </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. Mass spectrometry analysis and PCR sequencing revealed the 45 kDa antigen<br>to be a major capsid protein of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phage SE-W109. In conclusion, we report the protocol for generating a<br>polyclonal antibody against a </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">phage and that the main antigen recognized by antiserum is a major capsid<br>protein. The identified antibody will be used for further development of a phage-based method for detection of<br></span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">contamination in food samples.</span> </p>}, number={2}, journal={The Thai Journal of Veterinary Medicine}, author={Sangwiman, Anucha and Phothaworn, Preeda and Muangsombut, Veerachat and Chanprasert, Chutima and Chaichana, Chartchai and Tangjittipokin, Watip and Korbsrisate, Sunee}, year={2019}, month={Jun.}, pages={121–129} }