@article{Surachetpong_Nantakhruea_Inthasaeng_Lekcharoensuk_2018, title={ERK MAPK signaling pathway regulates porcine circovirus type 2 replication in peripheral blood mononuclear cells}, volume={48}, url={https://he01.tci-thaijo.org/index.php/tjvm/article/view/138028}, abstractNote={<p><span class="fontstyle0">Porcine circovirus type 2 </span><span class="fontstyle2">(</span><span class="fontstyle0">PCV2</span><span class="fontstyle2">) </span><span class="fontstyle0">is one of the most important swine viral diseases in Thailand and worldwide</span><span class="fontstyle2">.<br></span><span class="fontstyle0">While the interaction between PCV2 and pig immune system has been largely explored, the mechanisms by which<br>PCV2 replicates in porcine immune cells and alters host cellular signaling pathways remain incomplete</span><span class="fontstyle2">. </span><span class="fontstyle0">In this study,<br>extracellular regulated kinase </span><span class="fontstyle2">(</span><span class="fontstyle0">ERK</span><span class="fontstyle2">) </span><span class="fontstyle0">phosphorylation form was shown to slightly increase in PCV2</span><span class="fontstyle2">-</span><span class="fontstyle0">infected peripheral<br>blood mononuclear cells </span><span class="fontstyle2">(</span><span class="fontstyle0">PBMCs</span><span class="fontstyle2">) </span><span class="fontstyle0">at 48 hr post infection</span><span class="fontstyle2">. </span><span class="fontstyle0">To demonstrate the role of ERK MAPK signaling, PBMCs<br>were divided into three groups including control, virus, and virus with the mitogen</span><span class="fontstyle2">-</span><span class="fontstyle0">activated protein kinase </span><span class="fontstyle2">(</span><span class="fontstyle0">MEK</span><span class="fontstyle2">)<br></span><span class="fontstyle0">specific inhibitor, U0126, to prevent ERK phosphorylation</span><span class="fontstyle2">. </span><span class="fontstyle0">U0126 at 10 </span><span class="fontstyle3">µ</span><span class="fontstyle0">M completely inhibited ERK activation in the<br>porcine PBMCs at 48 and 72 hr</span><span class="fontstyle2">. </span><span class="fontstyle0">Interestingly, the inhibition of ERK MAPK significantly impaired PCV2 replication in<br>the PBMCs</span><span class="fontstyle2">. </span><span class="fontstyle0">In summary, our results unraveled the role of ERK MAPK signaling during PCV2 replication in porcine<br>immune cells</span><span class="fontstyle2">. </span><span class="fontstyle0">These findings provide important information on how PCV2 interacts with host cells and influences host<br>immune response</span><span class="fontstyle2">.</span> </p>}, number={2}, journal={The Thai Journal of Veterinary Medicine}, author={Surachetpong, Win and Nantakhruea, Sunaree and Inthasaeng, Phiangfhun and Lekcharoensuk, Porntippa}, year={2018}, month={Aug.}, pages={171–178} }