Pulp-dentin Complex Response to RU-HBM1, a Novel Resin Modified Glass Ionomer Cement Prototype, in Deep Cavity Preparation of Porcine Teeth

Abstract

The objective of this study was to evaluate the response of the pulp-dentin complex to Research Unit-Herbal Medicine, Biomaterial and Material 1 (RU-HBM1), a resin-modified glass ionomer (RMGI) cement prototype, in deep cavity of porcine teeth compared with commercial RMGIs. Six 14-week-old pigs were used in this study. Deep class V cavity was created on the buccal surface of canine, premolar, and molar teeth. The teeth were randomly lined with the design cement bases: RU-HBM1, Vitrebond (VB), or GC-Gold Label Light-Cured Universal Restorative (GC). After that, the cavity walls were treated with 30% phosphoric acid and washed. Then, a bonding agent was applied and the cavities were restored using light-cured resin-based material (Filtek Z250XT, 3M ESPE). The teeth were extracted at 7, 30, and 70 days post-operation, sectioned and stained for histopathological evaluation. Data were collected and analyzed using SPSS program. It was found that the RU-HBM1, VB, and GC groups had no inflammatory reaction at all time points of observation except one sample in the VB group had mild reaction at day 7. Reactionary dentin formation was detected in all groups at 30 and 70 days post-treatment. Normal pulp tissue pattern and cellular organization were observed in all groups at all time points of observation. There was no significant difference in the overall histopathological scores of the RU-HBM1, VB, and GC groups at all time points of evaluation (p>0.05). In conclusion, RU-HBM1, a novel resin-modified glass ionomer cement prototype, is biocompatible for deep cavity prepared in intact teeth.