Foal Deciduous Teeth Stem Cells Enhance Wound Healing in Rabbit Wound Model

Abstract

The purpose of this study was to evaluate the potential use of deciduous teeth stem cells from horses for wound healing in animal wound model. This study assessed the efficacy and safety of foal deciduous teeth stem cells (fDSCs) administration in rabbit excisional wound model. Deciduous teeth stem cells were harvested from horses and full thickness excisional wound was made on rabbits by surgical removal of epidermis and dermis to the depth of subcutaneous fat. New Zealand White rabbits (n=8) were divided into a control group (n=4) and a treatment group (n=4). Multiple injections of fDSCs suspension were intravenously administered into the rabbits with excisional wounds in the treatment group. Wound sizes were recorded on days 3, 5, 7, 10, 14, 21 and 28. The administration of fDSCs enhanced wound healing in the rabbit wound model. The scar sizes were significantly smaller compared with the control group (p<0.05). The rabbits exhibited immediate recovery with no complications. The transplantation of fDSCs provided good choice in terms of wound regeneration and healing. Therefore, our results suggested that fDSCs might provide a new approach to clinical treatment of wounds in veterinary patients, especially in horses in the future.