Alteration in Serum Matrix Metalloproteinase-9 Gelatinase Activities in Lymphoma-Bearing Dogs with Chemotherapies

Abstract

Matrix metalloproteinase-2 and -9 (MMP-2 and -9), gelatinases among MMPs, are associated with cell migration, invasion, metastasis and angiogenesis in human solid tumor. The prognostic value of gelatinases in canine lymphoma remains equivocal. To evaluate the relation between serum gelatinases and canine lymphoma, gelatin zymography was used to detect the levels of serum MMP-2 and MMP-9 gelatinase activities in lymphoma-bearing dogs. In this retrospective study, twenty-four canine patients with different subtypes of lymphoma were identified according to the histopathogical examination, and 10 healthy dogs were included as control. Serum samples from the lymphoma-bearing dogs were collected before and after chemotherapy. Results showed that the MMP-9 levels increased in the lymphoma-bearing dogs (median: 199.37, interquartile difference: 37.67) and decreased after a complete response was achieved (median: 140.64, interquartile difference 48.62); however, the MMP-2 levels among the controls and lymphoma-bearing dogs with treatment were similar (before: median: 138.42, interquartile difference: 35.7; complete response: median: 119.26, interquartile difference: 39.03). Both MMP-2 and MMP-9 levels were higher in T-cell lymphoma than in B-cell lymphoma patients. Compared with MMP-2, changes in the level of serum MMP-9 gelatinase activity could be a prognostic factor in canine lymphoma.