In Vitro Screening of Crude Extracts from Thai Plants in the Moraceae and Leguminosae Families on Proliferation of Swine Lymphocyte and Maturation of Monocyte-Derived Dendritic Cell

Abstract

In this study, nine crude extracts from Thai plants in the Moraceae and Leguminosae families were screened for their immunoadjuvant activities on the proliferation of swine lymphocytes and the maturation of monocyte-derived dendritic cells (MoDCs). The study demonstrated that the total phenolic content was significantly higher in the extract from Artocarpus gomezianus (264.17±6.5 mg of gallic acid equivalents/gm of extract) than in the extracts from the other plants. The crude extract from the root bark of A. gomezianus significantly increased the proliferative response of peripheral blood mononuclear cells measured using both MTT and CFSE assays (p < 0.05). In addition, the A. gomezianus extract induced the maturation of MoDCs through major histocompatibility (MHC) Class II and CD80 expression. Constituents of phenolic compounds, in particular tannins and flavonoids in the gamma-benzopyrone nucleus group, may be important for the immunoadjuvant effect. The results of this study suggest that the extract from A. gomezianus have immunoadjuvant activities that may be developed as vaccine adjuvants.