THE APPLICATION OF A RT-PCR TECHNIQUE FOR THE DIAGNOSIS OF CANINE DISTEMPER USING PERIPHERAL BLOOD MONONUCLEAR CELLS

Authors

  • Weerawan Tiwananthakorn
  • Sirichai Junsuwanaruk
  • Achariya Sailasuta
  • Anudep Rungsipipat
  • Somporn Techangamsuwan
  • Sapasiri Siripornprasert
  • Susumu Tateyama
  • Ryoji Yamaguchi
  • Kazuyuki Uchida

Keywords:

Canine distemper, peripheral blood mononuclear cells, RT-PCR

Abstract

The application of a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) on 37
canine distemper cases was performed by the amplification of the nucleo-capsid gene derived from the canine distemper virus or CDV-infected mononuclear cells (PBMCs). The animals were divided into 2 groups according to their clinical signs. The first group of animals showed signs of distemper, such as respiratory and/or digestive signs. The second group showed the same signs as first group but with nervous involvements. The findings revealed that 74.1% (20/27) of group 1 tested positive with the RT-PCR assay compared to 60% (6/10) of group 2. These results suggested that RT-PCR is a useful early diagnostic tool for CDV infected animals.

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How to Cite

Tiwananthakorn, W., Junsuwanaruk, S., Sailasuta, A., Rungsipipat, A., Techangamsuwan, S., Siripornprasert, S., Tateyama, S., Yamaguchi, R., & Uchida, K. (2015). THE APPLICATION OF A RT-PCR TECHNIQUE FOR THE DIAGNOSIS OF CANINE DISTEMPER USING PERIPHERAL BLOOD MONONUCLEAR CELLS. The Thai Journal of Veterinary Medicine, 32(3), 71–81. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/37919

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Original Articles