SERUM BONE-SPECIFIC ALKALINE PHOSPHATASE OF DOGS WITH VARIOUS BONE CONDITION

Abstract

Serum total and bone alkaline phosphatase (TAP and BAP) including Ca and P were measured in 6 dogs with osteoporotic bone and 10 dogs with less than a week fracture presented to the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The serum chemistry and ulnar radiographs were evaluated in plaster casting 9 normal experimental dogs before and after ulnar osteotomy on weeks 1, 2, 4, 6, 8, 10,
12, 14 and 16, postoperation. The 9 normal experiment dogs had the average (±SE) of 97.7(±19.1) U/L TAP, 23.3(±21.4) U/L BAP, 9.6(±0.4) U/L Ca and 5.4(±0.3) U/L P. Serum TAP, BAP, Ca ,and P of the osteoporotic dogs were significantly (p<0.05) higher while Ca to P ratio was lower, than those of the normal dogs. During bone healing, serum TAP and BAP had increased since the first postoperative week. Increased TAP reached the highest level at the 6th postoperative week and decreased to the normal preoperative level during the 16th week. TAP at the 2nd, 4th, 6th weeks were significantly (p<0.05) higher than those measured before osteotomy. BAP at every interval after osteotomy were significantly (p<0.05) higher than the preoperative levels. BAP reached the highest level at the 4th week and remained the high level until the 12th week before decreasing to the level close to the preoperative level. Ca, P and Ca:P ratio were normal throughout 16 weeks, except at the 6th week when the Ca level was significantly decreased (p<0.05). From the finding that BAP increase when there is bone abnormality. BAP might be used as an indicator for the diagnosis, treatment follow up and research of bone diseases.