PCR-RFLP ANALYSIS IN AN OPEN READING FRAME 5 OF VARIANTS OF PRRSV ISOLATED IN THAILAND

Authors

  • Nusara Punprapa
  • Anudep Rungsipipat
  • Amornrat Tatsanakit
  • Piya Wongyanin
  • Roongroje Thanawongnuwech

Keywords:

Porcine Reproductive and Respiratory Syndrome Virus, restriction fragment length polymorphism, open reading frame 5

Abstract

Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the
result of the introduction of a new variant into the endemic area or by local evolution.

Downloads

How to Cite

Punprapa, N., Rungsipipat, A., Tatsanakit, A., Wongyanin, P., & Thanawongnuwech, R. (2015). PCR-RFLP ANALYSIS IN AN OPEN READING FRAME 5 OF VARIANTS OF PRRSV ISOLATED IN THAILAND. The Thai Journal of Veterinary Medicine, 34(2), 47–56. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/37305

Issue

Section

Original Articles