A COMPARATIVE STUDY ON THE ISOLATION OF UREAPLASMA DIVERSUM FROM THE VULVAE OF THE CATTLE, USING THREE DIFFERENT CULTURE MEDIA

Abstract

The objective of the present study was to investigate the optimum culture media for Ureaplasma diversum. The study used 3 culture media, TR broth and differential A 7 agar (1), Boston broth and differential A7 agar (2) and differential A7 agar (3). The samples were collected from the vulvae of 15 cows that had a reddish, mucosal, vulva lesion and 15 cows without the lesion. A liquid culture media was prepared using the serial, tenfold, dilutionmethod to avoid contamination from other microflora. The type of U. diversum was determined using A7 agar mixed with manganese sulfate. Positive results were observed as brown colonies with a variety of shapes, such both round and smooth. The study revealed that U. diversum could be cultured in media 1, 2 and 3 from 33.3%, 53.3% and 26.7%, respectively, from cattle with lesions of the disease. U. diversum could also be cultured in media 1, 2 and 3 from 13.3% of cattle without lesions of the disease. Using media 2, the proportion of U. diversum cultured was higher in cattle with the lesion, compared with cattle without the lesion (p<0.05). In conclusion, Boston broth with differential A7 agar (media 2) was recommended as the prefered medium for culturing U. diversum.