Isolation of Actinobacillus pleuropneumoniae Serotype 15-like Strain from a Porcine Tonsil in Thailand: A Case Report

Abstract

An isolate of Actinobacillus (A.) pleuropneumoniae from porcine tonsil samples was identified as serotype 15-like strain, which was previously isolated in Australia, North America and Japan. It was characterized by biochemical testing performed for Actinobacillus sp. and confirmed as A. pleuropneumoniae by PCR and nucleotide sequencing of 16s rDNA. The isolate contains apxIBD, apxIICA, apxIIIBD, apxIIICA and apxIVA gene. The result of PCR-REA of apxIVA method was identical as A. pleuropneumoniae serotype 15 strain HS143 reference strain. Therefore this isolate is proposed as A. pleuropneumoniae serotype 15- like strain and the first report of A. pleuropneumoniae serotype 15-like strain in Thailand. This finding is important for epidemiological investigations of A. pleuropneumoniae infections and it may support the selection of an appropriate vaccine formulation.