Prevalence of the Cellular and Molecular Antimicrobial Resistance against E. coli Isolated from Thai Broilers
Keywords:Antimicrobial resistant gene, Escherichia coli, poultry, sensitivity test
The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed by disk diffusion tests, the 86.4% SHV, 100% sul1 and 100% dhfrV genes were found from PCR techniques. However, no antimicrobial resistance both in the cellular and molecular
levels of gentamicin and chloramphenical were found.