Isolation and Characterization of Potential Probiotic Bacteria Suitable for Mollusk Larvae Cultures

Authors

  • Ruth Escamilla-Montes
  • Antonio Luna-González
  • Ma. del Carmen Flores-Miranda
  • Píndaro Álvarez-Ruiz
  • Jesús Arturo Fierro-Coronado
  • Ana Claudia Sánchez-Ortiz
  • José Ávila-Leal

Keywords:

bacilli, lactic acid bacteria, larvae, mollusks, probiotics

Abstract

Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and some resistance to antibiotics. The isolates were identified as Bacillus licheniformis (BCR 4-3) and Leuconostoc mesenteroides (BALCR 5-2 and BALOR 2-3). The strains showed basic characteristics to be used as probiotics in mollusk larvae cultures.

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How to Cite

Escamilla-Montes, R., Luna-González, A., Flores-Miranda, M. del C., Álvarez-Ruiz, P., Fierro-Coronado, J. A., Sánchez-Ortiz, A. C., & Ávila-Leal, J. (2015). Isolation and Characterization of Potential Probiotic Bacteria Suitable for Mollusk Larvae Cultures. The Thai Journal of Veterinary Medicine, 45(1), 11–21. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/32129

Issue

Vol. 45 No. 1 (2015): March; 80 Years CU VET "Responsible for Lives"

Section

Original Articles