Generation of tail bearing sperm-like cells from in vitro spermatogenesis of farming goat testis

Authors

  • Rakesh Bhaskar
  • Bijaylaxmi sahoo
  • Sung Soo Han
  • Mukesh Kumar Gupta

Keywords:

Air-liquid inter-phase, In vitro spermatogenesis, Organ culture, Germ-line stem cells, Goat, Differentiation

Abstract

In vitro spermatogenesis offers a therapeutic modality for the treatment of male infertility associated with pre-or post-meiotic barriers to the spermatogenesis process. The present study reports the production of tail-bearing sperm-like cells from in vitro spermatogenesis of farming goat testis in an air-liquid inter-phase culture. The testicular fragments (1-2 mm3) were placed on agarose scaffolds at air-liquid interphase and cultured in vitro for 60 days. Proliferating germ cell colonies were visible after 45-55 days of culture. Differentiation of cells to spermatogonia and spermatocytes was observed from the 40th day onwards. Tail bearing sperm-like elongated cells, with apical acrosome, were detected from the 45th day onwards. The differentiating germ-line cells were confirmed by stage-specific expression of positive markers (VASA, DAZL, SYCP1, SYCP3, C-Kit, POU5F1 and SOX-2). Histological sections of the cultured testicular tissue further confirmed the presence of tail-bearing sperm-like cells in the lumen of seminiferous tubules which was absent in non-cultured tissue. In conclusion, our study suggests that the air-liquid interphase organ culture might be useful in vitro generation of sperm in farming goats. The study could have a significant impact on the livestock management of the farmers and could improve their dependency on orthodox and natural breeding management.

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Published

2022-08-25

How to Cite

Bhaskar, R. ., sahoo, B. ., Han, S. S. ., & Gupta, M. K. . (2022). Generation of tail bearing sperm-like cells from in vitro spermatogenesis of farming goat testis. The Thai Journal of Veterinary Medicine, 52(3), 473–484. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/258306

Issue

Section

Original Articles