Seasonal and antimicrobial resistance distributions of Salmonella isolated from pork, beef and chicken meat in Vientiane Capital, Lao PDR

Abstract

The objectives in this study was to investigate the prevalence, seasonal variation and antimicrobial resistance of Salmonella isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR. During May 2018 to April 2019, 445 swab samples were randomly collected from pork (n = 258), beef (n = 81) and poultry meat (n = 106) in three seasons at three retail big markets in Vientiane capital, Lao PDR, Thongkhankham, Lao-Aussi, and Nongchan markets. All samples were isolated and identified for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 procedure. The isolates were tested for antimicrobial susceptibility using the Kirby-Bauer method. The prevalence of Salmonella contamination isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR were 49.2%, 61.7% and 61.3% respectively. Significantly higher number of Salmonella spp. was observed in beef and chicken meat during rainy season. The most common serotype in pork, beef and chicken meat was S. Rissen (37.0%), S. Derby (10.8%) and S. Corvallis (38.0%), respectively. The highest resistance was found for TET (72.3%), followed by AMP (62%), SXT (51.7%) and CHL (26%). Hygiene and sanitation of slaughterhouse and retail markets should be concerned to control and prevent of Salmonella spp. contamination along the food supply chain.