Cryopreservation methods of Thai native chicken (Pradu-hangdum) semen: effect of different freezing containers on motion and kinetic parameters of frozen-thawed sperm

Abstract

Artificial Insemination (AI) programs using qualified semen are designed to revolutionize the poultry farming sector by targeting genetic improvement along with appropriate health strategies. Hence, a prolonged preservation method of quality sperm conserves is important for successful AI outcomes. The objective of this study was to compare the effects of different type of containers; straw, cryovial tubes and pellet on the quality of the frozen-thawed Thai native chicken (Pradu-hangdum) semen. High-quality pooled semen was collected and divided into 4 groups with 12 replicates: semen preserved with BHSV extender in 1) 0.25 mL straw, 2) 0.5 mL straw, 3) 2 mL cryovial tube, and 4) semen pellet in 2 mL cryovial tube. All containers were stored in a liquid nitrogen tank for 1 week and the frozen-thawed sperm were subsequently analyzed. The results indicated that the mean of all motion parameters including total motility, progressive motility, progressive fast motility and progressive slow motility, and some kinetic parameters such as DCL, DSL, ALH, BCF, and HAC of the frozen-thawed sperm preserved in cryovial tube had significantly higher values compared to other groups (P < 0.05). In conclusion, semen freezing process in cryovial tubes had the least effect on decreasing the qualities of frozen-thawed sperm than other methods. Furthermore, fertility rates related to chicken semen preservation should be further evaluated to provide valuable knowledge to farmers.