Fluid resuscitation with Ringer’s and trometamol-balanced solutions in a rat septic model

Abstract

Resuscitation solution is one of the main interventions provided for septic shock patients, although there is no general consensus regarding the optimal type of solution. Therefore, a trometamol-balanced solution (TBS) was designed as a resuscitation solution. The aim of this study is to assess the effects of TBS versus Ringer’s solution (RS) in a rat septic model. Septic shock was induced in 16 male Wistar-Kyoto rats through lipopolysaccharide (LPS) induction; these rats were assigned at a ratio of 1:1 to RS and TBS groups. Blood examinations were performed using an Abbott i-STAT analyzer with CG4+ (for pH, pressure of carbon dioxide, pressure of oxygen, total carbon dioxide, bicarbonate, base excess, oxygen saturation, and lactate) and CG6+ (for sodium, potassium, chloride, blood glucose, blood urea nitrogen, hematocrit, and hemoglobin) and enzyme-linked immunosorbent assay kits (calcium, magnesium, creatinine, aspartate aminotransferase, alanine aminotransferase, bilirubin, and albumin) during the septic state. The biochemical parameters, electrolytes, and blood gas parameters implied similar trends and the majority of data showed no considerable changes between groups after LPS-induced septic shock. However, the pH values of the TBS group were more stable compared with those of the RS group. This indicated that TBS might be more effective in metabolic acidosis alleviation. In summary, TBS is safe and feasible in this study and may offer advantages in septic shock cases without hepatic diseases. Future research will focus on applying TBS in other different animal disease models and the clinical usage of TBS.