Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy

  • Pornphimon Metheenukul
  • Kanjana Thumanu
  • Thanit Metheenukul
Keywords: canine erythrocytes, dog erythrocyte antigen, DEA 1.1, FTIR spectromicroscopy, trypsin

Abstract

A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopy was used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+ canine erythrocytes. Production of canine DEA 1.1- erythrocytes was achieved by treating DEA 1.1+ red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1- erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR microspectroscopy data suggested that the trypsin treated dog erythrocytes were possible for use as universal blood.

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Published
2021-03-22
How to Cite
Metheenukul, P., Thumanu, K., & Metheenukul, T. (2021). Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy. he hai ournal of eterinary edicine, 51(2), 321-329. etrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/248716
Section
Original Articles