Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy

Authors

  • Pornphimon Metheenukul
  • Kanjana Thumanu
  • Thanit Metheenukul

Keywords:

canine erythrocytes, dog erythrocyte antigen, DEA 1.1, FTIR spectromicroscopy, trypsin

Abstract

A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopy was used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+ canine erythrocytes. Production of canine DEA 1.1- erythrocytes was achieved by treating DEA 1.1+ red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1- erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR microspectroscopy data suggested that the trypsin treated dog erythrocytes were possible for use as universal blood.

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Published

2021-03-22

How to Cite

Metheenukul, P. ., Thumanu, K. ., & Metheenukul, T. . (2021). Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy. The Thai Journal of Veterinary Medicine, 51(2), 321–329. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/248716

Issue

Section

Original Articles