Development and validation of a diagnostic system for the detection of specific antibodies and antigens against Porcine Epidemic Diarrhea Virus

  • Bo Dong
  • Chuanjiang Yang
  • Haiyan Zhang
  • Kesong Jiang
  • Xiaoyan Yang
Keywords: indirect ELISA, N protein, porcine epidemic diarrhea virus, sandwich ELISA

Abstract

Porcine epidemic diarrhea virus (PEDV) is highly pathogenic and infects suckling piglets, thereby imposing a major
burden on the swine industry all over the world. The aim of this study is to develop two ELISA systems for the detection
of antigens and antibodies against PEDV in pigs. The PEDV N recombinant protein was expressed and was used as the
coating antigen to establish an indirect ELISA for detecting PEDV-specific antibodies in pig serum. Moreover, MAb
was induced with the recombinant N protein and used for sandwich ELISA to specifically test for PEDV. The
specificities of the two ELISAs were validated by comparing the results with other viruses and the reproducibility was
evaluated by intra-assay or inter-assay variation. The diagnostic sensitivity and specificity also were evaluated by the
receiver operating characteristic (ROC) curve. The two ELISAs both indicated acceptable reproducibility. Neither
ELISA cross-reacted with other viruses. ROC curve analysis revealed 100.0% sensitivity and 95.2% specificity of the
iELISA, 100.0% sensitivity and 90.0% specificity of the sELISA. Consequently, this work indicates that these two ELISAs
can be used clinically to diagnose PEDV.

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Published
2020-12-01
How to Cite
Dong, B., Yang, C., Zhang, H., Jiang, K., & Yang, X. (2020). Development and validation of a diagnostic system for the detection of specific antibodies and antigens against Porcine Epidemic Diarrhea Virus. he hai ournal of eterinary edicine, 50(4), 503-510. etrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/246318
Section
Original Articles