Effect of fermented red radish (Raphanus sativus L) in carbon tetrachloride-induced liver injury in rats

Abstract

In this study, the possible protective effect of red radish containing total phenolic content and antioxidant
components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity,
triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were
evaluated in CCl4 induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each
group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw);
while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was given daily by intragastric administration
to group II and group III, respectively for seven consecutive days. Carbon tetrachloride (CCl4) diluted 1:1 ratio in corn
oil and was given (1.5 mL/kg) to the rats in group IV by intraperitoneal injection. Group V and VI received FRR at a
dose of 250 mg/kg or 500 mg/kg, respectively for seven consecutive days prior to CCl4 injection. Pretreatment with
FRR at 250 mg/kg and 500 mg/kg followed by CCl4 exposure had no ameliorative effect on the histopathological
changes in the liver. It is thought that FRR application has a partial antioxidative effect by decreasing the biochemical
and lipid peroxidation levels in the control group but this is not effective in the treatment of damage to liver tissue. In
conclusion, it has been concluded that new investigations should be done to determine the healing effects of red radish
on fermented tissues using different doses to give the best results without any side effects.