Isolation and identification of Klebsiella aerogenes from bee colonies in bee dysbiosis

Abstract

This article presents modern methods for obtaining and isolating pure culture from bee colonies (from honeycombs
with infected broods and contaminated by faeces of bees), and the identification and indication of pathogenic bacteria
of Klebsiella aerogenes species in honey bees during the winter, spring, summer and the autumn. The purpose was to
isolate and identify the pure culture of enterobacteria from the pathological material of diseased bee colonies. The study
of their basic biological properties for the possible use of the obtained strains, which should be used as indicator
bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis
of bees was undertaken. The etiological factor for the origin of the collapse of bee colonies belongs to the Family
Enterobacteriaceae, the genus being Klebsiella – Klebsiella aerogenes (previously named Enterobacter aerogenes),
which was established by its cultural properties (Endo – light pink, small, flat, mucous, matte colonies; Levin – light
pink, flat, mucous, matte colonies); by morphological properties (short, sticks, small, placed together and singly,
without capsules, mobile); by tinctorial (gram negative) properties and by results of biochemical typing of
microorganisms (Kligler medium: glucose – negative, acid-gas H2S negative; Simons medium – positive, acetate Na –
positive, malonate Na – negative; Phenylalanine – negative: Indole –negative; Urease – negative), which were isolated
from the intestines of bees and their faeces from the frames of bee colonies.