Pulmonary squamous cell carcinoma in a captive clouded leopard (Neofelis nebulosa)

Abstract

A female, 5-year-old clouded leopard died three days after showing clinical signs of lethargy and loss of appetite.
Macroscopically, multiple, firm, nodular masses were found randomly in the caudal lobes of the lungs. There were no
metastatic lesions on the skin. Histopathological examination demonstrated multifocal trabeculae and a nest of large,
variable sized of squamous epitheliums. Multifocal keratin pearls were also randomly seen in the masses. No metastasis
was found in the other internal organs. Immunohistochemically revealed that the neoplastic cells were
immunoreactivity positive for cytokeratin, while negative for vimentin. Based on the histopathology and
immunohistochemistry, pulmonary squamous cell carcinoma (SCC) was diagnosed.