Screening antimicrobial properties against mastitis pathogens of turmeric extract after combination with various antiseptics

Abstract

The aim of this study was to determine the screening for antimicrobial properties against mastitis pathogens of
turmeric extract in combination with various antiseptics. The antibacterial effects of turmeric extract in combination
with various antiseptics (5% povidone iodine, 0.5% v/v hydrogen peroxide (H2O2), 0.5% v/v chlorine (Cl2), and 0.5%
v/v chlorhexidine) were determined using the agar well diffusion method. Results showed that additional turmeric
extract had significantly decreased the antimicrobial activities of either Cl2 or chlorhexidine against almost all mastitis
pathogens, except S. agalactiae for Cl2 and gram negative bacteria for chlorhexidine. In contrast, no negative effect was
found between turmeric extract and H2O2 against most mastitis pathogens. In addition, a positive antimicrobial effect
of turmeric extract and H2O2 against other Streptococcus spp was found. In conclusion, H2O2 was the only antiseptic that
can combine with turmeric extract and might support the advantages of the combination to improve wound healing
and the antiseptic properties of H2O2 for further development for a future antiseptic product for teat dipping, especially
for cows with teat end score damage.