Gene expression profiling of NGF, BDNF, NT-3 and TrkB receptor in the development of prehierarchical follicles of Zi Geese (Anser cygnoides)

Authors

  • Cornelius Tlotliso Sello
  • Chang Liu
  • Yujian Sui
  • Hui Yang
  • Ziqiu Wang
  • Hongtao Lu
  • Jingtao Hu
  • Yongfeng Sun

Keywords:

Folliculogenesis, neurotrophins, RT-qPCR, Western blot

Abstract

The expression patterns of neurotrophins (NTs) at the gene and protein level including nerve growth factor (NGF),
brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and TrkB receptor were investigated in the
prehierarchical follicles of Zi Geese by RT-qPCR and Western blotting analysis. The mRNA levels of NGF, BDNF, NT-
3 and TrkB receptor increased significantly in the small white follicles (SWF), and more interesting by BDNF and NT-
3 transcripts were not detectable in small yellow follicles (SYF) marked as the last stage of prehierarchical ovarian
development before transition into preovulatory follicles. Western blot revealed the presence of all the target
neurotrophins and high-affinity receptor at the protein level with varying predominance at each stage of follicle
classifications. These results indicate that NGF, BDNF, NT-3, and TrkB are implicated in the folliculogenesis and may
have an influential effect during the development and growth of the Zi Geese reproductive system.

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Published

2019-11-27

How to Cite

Tlotliso Sello, C., Liu, C., Sui, Y., Yang, H., Wang, Z., Lu, H., Hu, J., & Sun, Y. (2019). Gene expression profiling of NGF, BDNF, NT-3 and TrkB receptor in the development of prehierarchical follicles of Zi Geese (Anser cygnoides). The Thai Journal of Veterinary Medicine, 49(3), 235–241. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/230619

Issue

Vol. 49 No. 3 (2019): September

Section

Original Articles