A simple and rapid method for the extraction of genomic DNA from piglet tails without using proteinase K

Authors

  • Nathamon Yimpring
  • Komkrich Teankum
  • Prapat Suriyaphol
  • Nutthee Am-in
  • Gunnaporn Suriyaphol

Keywords:

ceramic beads, genomic DNA extraction, homogenization, piglet tails, vortex mixer

Abstract

Genomic DNA (gDNA) is a prerequisite for genomic research. However, the collection of blood samples from
young animals for gDNA extraction is not practical. Since piglet tail docking is routinely performed in pig farms to
prevent injury from tail biting, docking tails served as a practical source for gDNA in piglets. However, extraction of
gDNA from piglet tails is difficult because of inelasticity and cartilage. The purpose of this study was to develop a rapid
and simple method of isolating gDNA from piglet tails for downstream molecular purpose. Chopped piglet tails with
ceramic beads in lysis buffer were vortex-homogenized prior to DNA extraction with a general tissue gDNA extraction
kit without a long incubation period in proteinase K. Obtained gDNA was used for polymerase chain reaction
amplification of the microsatellite loci, reflecting the quality of gDNA. In conclusion, we reveal the practical tools for
gDNA extraction from piglet tails for molecular analysis.

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Published

2019-05-16

How to Cite

Yimpring, N., Teankum, K., Suriyaphol, P., Am-in, N., & Suriyaphol, G. (2019). A simple and rapid method for the extraction of genomic DNA from piglet tails without using proteinase K. The Thai Journal of Veterinary Medicine, 49(1), 65–69. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/198930

Issue

Vol. 49 No. 1 (2019): March

Section

Original Articles