Dual infection of a Thai isolate HP-PRRSV and the pdmH1N1 2009 SIV in weanling pigs


  • Nattaphong Sangpratum
  • Roongtham Kedkovid
  • Yonlayong Woonwong
  • Chaitawat Sirisereewan
  • Sawang Kesdangsakonwut
  • Roongroje Thanawongnuwech
  • Jirapat Arunorat


co-infection, highly pathogenic PRRSV, pandemic H1N1 2009, pathogenesis, swine influenza virus


After the introduction of the pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in Thai swine farms in
2009, a mild clinical outcome was observed and its role in porcine respiratory disease complex (PRDC) is questionable.
A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in Thai
swine farms, recently. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected
with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma
spp. commercial farm were randomly divided into 4 groups; Negative, PRRSV, the SIV and Co-infection groups. Pigs
in the PRRSV and Co-infection groups were challenged intranasally with the Thai isolate HP-PRRSV at 0 day post HPPRRSV challenge (dphc) and pigs in the SIV and Co-infection groups were inoculated intratracheally with the
pdmH1N1 SIV at 6 dphc. The 1st and 2nd necropsies were at 2 and 4 days post SIV challenge (dsc). A comparison of
the lung lesion scores between the PRRSV and Co-infection groups at 2 dsc showed no statistical difference, but at 4
dsc, the lung lesion scores of the Co-infection group had higher average scores than those of the PRRSV group.
Similarly, the SIV viral load in the lung of the Co-infection group a showed higher viral load at 4 dsc, compared to the
only SIV-infected group. The results suggest that low pathogenic pdmH1N1 SIV could enhance the lung severity when
co-infected with the HP-PRRSV leading to PRDC mimicking the field scenarios.




How to Cite

Sangpratum, N., Kedkovid, R., Woonwong, Y., Sirisereewan, C., Kesdangsakonwut, S., Thanawongnuwech, R., & Arunorat, J. (2019). Dual infection of a Thai isolate HP-PRRSV and the pdmH1N1 2009 SIV in weanling pigs. The Thai Journal of Veterinary Medicine, 49(1), 71–79. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/193699



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