Buccal Swab as a Source of Noninvasive Technique for Genomic DNA Collection in Felidae

Authors

  • Janjira Phavaphutanon Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140,
  • Sudtisa Laopium Kamphaeng Saen Veterinary Diagnosis Center, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140
  • Kawin Nanklang Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140
  • Kaikanoke Sirinarumitr Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140,
  • Kornchai Kornkaewrat Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140,
  • Anuchai Pinyopummin Department of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140
  • Jitrakorn Viriyarumpa Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140,
  • Piyawan Suthanmapinunt Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140,
  • Narathip Vorawattanatham Veterinary, Conservation and Research Section, Animal Management Division, Chiang Mai Night Safari, Chiang Mai 50230

Keywords:

buccal swab, felid, genomic DNA, non-invasive technique

Abstract

This study was conducted to determine whether buccal cells obtained by swabbing could be a DNA source for genetic analysis, and to compare two preservative media, distilled water and Tris-EDTA (TE), pH 8.0. Buccal cell
samples were collected from 30 domestic cats (Felis catus), 4 captive fishing cats (Prionailurus viverrinus) and 8 captive tigers (Panthera tigris). The total concentration of DNA and purity were not statistically different between the preservative media. All samples could be graded as a minimum as high quality DNA (DNA found with A260/A280 ratio >2.0 or < 1.8). In addition, the quality of DNA extracted from both media was successfully amplified for PCR. However, when compared to whole blood samples, total DNA concentration from buccal swab was significantly
lower (p < 0.05) .Despite the decrease in DNA yield, buccal swab could be an alternative source for obtaining DNA for genetic analyses. This non-invasive, simple and inexpensive technique eases genetic studies in this family especially in domestic cat from which whole blood samples are difficult to collect.

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How to Cite

Phavaphutanon, J., Laopium, S., Nanklang, K., Sirinarumitr, K., Kornkaewrat, K., Pinyopummin, A., Viriyarumpa, J., Suthanmapinunt, P., & Vorawattanatham, N. (2013). Buccal Swab as a Source of Noninvasive Technique for Genomic DNA Collection in Felidae. The Thai Journal of Veterinary Medicine, 43(3), 455–460. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/12284

Issue

Vol. 43 No. 3 (2013): SEPTEMBER

Section

Short Communications