Genetic Diversity of Mycoplasma hyosynoviae Field Isolates in Thailand

Authors

  • Pacharee Thongkamkoon National Institute of Animal Health, Bangkok 10900, Department of Agriculture, Tokyo University of Agriculture, Tokyo, Japan
  • Sommai Yuwapanichsampan National Institute of Animal Health, Bangkok 10900,
  • Hideki Kobayashi National Institute of Animal Health, Tsukuba, Ibaraki 305-0856,
  • Pornpen Pathanasophon National Institute of Animal Health, Bangkok 10900,
  • Masato Kishima Diseases Investigation Center, Subang, West Java,
  • Koshi Yamamoto Department of Agriculture, Tokyo University of Agriculture, Tokyo,

Keywords:

genetic diversity, Mycoplasma hyosynoviae, PFGE, RAPD, Thailand

Abstract

Pulsed-field gel electrophoresis (PFGE) and Random amplified polymorphic DNA (RAPD) analyses were performed to compare techniques and to investigate genetic diversity as an epidemiological data of Thai M.
hyosynoviae isolates. A total of 42 isolates including a type strain S16 was typable and consisted of 39 different patterns by RAPD technique, whereas 37 isolates (97%) were typable and consisted of 22 different patterns by PFGE technique. Based on PFGE patterns, multiple clones of M. hyosynoviae were generally present in pig farms, whereas high genetic
heterogeneity of M. hyosynoviae among the pig farms was shown. No identical PFGE pattern between the pig farms was found except two farms that were located in the same province. This finding might indicate the distribution of the organism from the same source. Monitoring the genetic diversity of M. hyosynoviae strains using PFGE analysis should be useful to elucidate the epidemiology of M. hyosynoviae infections in Thailand.

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How to Cite

Thongkamkoon, P., Yuwapanichsampan, S., Kobayashi, H., Pathanasophon, P., Kishima, M., & Yamamoto, K. (2013). Genetic Diversity of Mycoplasma hyosynoviae Field Isolates in Thailand. The Thai Journal of Veterinary Medicine, 43(3), 359–365. Retrieved from https://he01.tci-thaijo.org/index.php/tjvm/article/view/12270

Issue

Vol. 43 No. 3 (2013): SEPTEMBER

Section

Original Articles