TY - JOUR AU - Katib, Somdet AU - Ruangrungsi, Nijsiri AU - Palanuvej, Chanida AU - Chareonsap, Piyarat Parinyapong AU - Rungsihirunrat, Kanchana PY - 2017/03/15 Y2 - 2024/03/29 TI - Macroscopic-Microscopic Characteristics and AFLP Marker for Identification of Tinospora Crispa and Tinospora Baenzigeri Endemic to Thailand JF - Journal of Health Research JA - J Health Res VL - 31 IS - 2 SE - ORIGINAL RESEARCH ARTICLE DO - UR - https://he01.tci-thaijo.org/index.php/jhealthres/article/view/79750 SP - 143-149 AB - <p style="text-align: justify;"><strong>Background:</strong> <em>Tinospora</em> <em>crispa</em> Miers ex Hook. F &amp; Thomson and <em>Tinospora</em> <em>baenzigeri</em> Forman were used in traditional medicine for treatment of antipyretic and various ailments. According to the similarity in their morphology and vernacular name, the identification was important for their effectiveness.</p><p style="text-align: justify;"><strong>Methods:</strong> Macroscopic-microscopic characteristics and AFLP marker of <em>Tinospora crispa</em> and <em>Tinospora baenzigeri</em> were evaluated.</p><p style="text-align: justify;"><strong>Results:</strong> Macroscopic determination revealed that <em>T. crispa</em> had more prominently tuberculate on stem than <em>T. baenzigeri.</em> The leaf shape of <em>T. crispa</em> was cordate but <em>T. baenzigeri </em>was cordate or reniform and showed a two node appearing at leaf base. However, both of them showed anomocytic stomata type but <em>T. crispa</em> presented only on abaxial epidermis whereas <em>T. baenzigeri </em>presented on both adaxial and abaxial epidermis. Leaf constant numbers of these two species showed individual value. A total of 476 AFLP bands ranging in size from 50 to 800 base pairs were generated from 5 AFLP primer combinations, of which 457 bands were polymorphic (96%). The primer combination of E+ACG/M+CTT produced the highest number (125 bands) of AFLP bands. The similarity index ranged from 0.472 to 0.934. The dendrogram generated from UPGMA indicated that<em> T. crispa</em> and <em>T. baenzigeri</em> were clearly separated from each other.<strong></strong></p><p style="text-align: justify;"><strong>Conclusion:</strong> Macroscopic-microscopic characteristics and AFLP marker could be used for identification of these 2 <em>Tinospora</em> species. The information provided from this study can be used for identification of medicinal plants.</p> ER -