@article{Nemidkanam_Chaichanawongsaroj_2017, title={Kaemperia parviflora Extract Inhibits Caspase-Dependent Apoptosis Stimulated by H. pylori Infection}, volume={31}, url={https://he01.tci-thaijo.org/index.php/jhealthres/article/view/104293}, abstractNote={<p><strong>Background: </strong>Apoptosis process is one of the major pathogenesis in gastric cancer causing by <em>Helicobacter pylori </em>infection. <em>Kaempferia parviflora</em> (KP) has been traditionally used for treating gastrointestinal tract disorders, which exhibited anti-<em>Helicobacter pylori</em>, anti-inflammatory and <br> anti-gastric ulcer. Our study aimed to evaluate the anti-apoptosis effects of KP extract in <em>H. pylori</em>-infected gastric epithelial cells.</p> <p><strong>Methods: </strong>The cytotoxic effect of KP extract was assessed by MTT assay in AGS cells at various concentrations and incubation times. The level of caspase-8 and caspase-3/7 activities and DNA fragmentation were determined by luminescence assays and ELISA, respectively.</p> <p><strong>Results:</strong> KP ethyl acetate extract significantly decreased AGS cells viability in dose and time-dependent manners, no cytotoxic effect was observed at 8 and 16 µg/ml of KP extract even 24 hours. <em>H. pylori</em> activated apoptosis mainly via caspase-3/7 more effectively than caspase-8. Interestingly, KP extract significantly inhibited both caspase-3/7 and caspase-8 in <em>H. pylori</em> infected and uninfected cells, although DNA fragmentation was not suppressed.</p> <p><strong>Conclusions:</strong> Thus, KP extract may be a prominent alternative therapy for gastric cancer induced by <em><br> H. pylori</em> infection through the inhibition of initiator caspase-8 and effector caspase-3/7 which play important roles in apoptosis pathway.</p>}, number={Suppl. 1}, journal={Journal of Health Research}, author={Nemidkanam, Variya and Chaichanawongsaroj, Nuntaree}, year={2017}, month={Nov.}, pages={s41-s49} }