@article{Arunrerkdeewong_Pattaradilokrat_Siripoon_Harnyuttanakorn_2017, title={Effect of Temperatures on the Parasitaemia of Plasmodium falciparum in vitro}, volume={31}, url={https://he01.tci-thaijo.org/index.php/jhealthres/article/view/104273}, abstractNote={<p><strong>Background:</strong> <em>Plasmodium falciparum </em>is the most important human malaria parasite. To further improve the cultivation and related techniques of this parasite, effect of temperatures on the <em><br> P. falciparum </em>cultivation, <em>in vitro</em>, was studied. The total, ring and non-ring stages of synchronized culture, at different temperatures, were determined to evaluate their effects on the growth and development of the parasites.</p> <p><strong>Methods:</strong> Cultures of <em>P. falciparum </em>were exposed to different temperatures, 4, 34, 37 and 40 °C. The effect of temperature was determined as percent parasitaemia of ring and non-ring stage. K1CB1, T9/94RC17 and 3D7 clones were used in this experiment.</p> <p><strong>Results:</strong> The growth pattern of  all 34 °C cultures were similar to control (37 °C) while the parasites were killed after 72 hours exposure at 4 and 40 °C. In 4 °C culture, the major population was mainly in ring stage. On the contrary, only the non-ring stage can be detected after 48 hours incubation at 40 °C.</p> <p><strong>Conclusion:</strong> At 4, 34 and 40 °C, the <em>P. falciparum</em> growth was lowered, compared with the control group at 37 °C. All parasites cannot be detected after 48 or 72 hours incubation at 4 and 40 °C. In 4 °C culture, most detected parasites were ring stage while in 40 °C, most detected parasites were non-ring stage. Stage preferences by different temperatures may applicable for synchronization technique improvement.</p>}, number={Suppl. 1}, journal={Journal of Health Research}, author={Arunrerkdeewong, Kanokwan and Pattaradilokrat, Sittiporn and Siripoon, Napaporn and Harnyuttanakorn, Pongchai}, year={2017}, month={Nov.}, pages={s1-s6} }