Scavenging Activities and Protective Effects of Syzygium Cumini (L.) Skeels on H2O2 Induce Oxidative Stress in Normal Human Peripheral Blood Mononuclear Cells

Authors

  • Lanchakon Chanudom School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand
  • Jitbanjong Tangpong School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

Keywords:

Protective effects, Scavenging capacities, Syzygium cumini (L.) Skeels

Abstract

Syzygium cumini (L.) Skeels is the most commonly used as medicinal plants on diabetes and anti-inflammation. Hence, effects of aqueous leaf extracts of Syzygium cumini (L.) Skeels (ASC) on scavenging activities and protective effects of H2O2- induced oxidative damage in human peripheral blood mononuclear cells (PBMC) were determined in this study. Total phenolic and flavonoid contents of ASC at 2,000 µg/ml were 694.44±3.64 µgGAE/ml and 8.27±0.10 µg quercetin/ml respectively which were correlated with the r2 value at 0.93. IC50 values of ASC on free radical, hydroxyl and superoxide were 87.79±0.85, 766.62±28.61 and 1,299±25.13 µg/ml respectively while the nitric oxide scavenging activities were not higher than 50% at the concentration of 2,000 µg/ml of ASC. The protective effects of ASC in H2O2-induced PBMC cell damages were significantly increased to 83.13±3.57–112.32±4.47 % when treated with 0.25–1 µg/ml of ASC after oxidative induced by 5 mM of H2O2 for 3 h. Suggest that ASC is a potential source of natural antioxidant properties and protective defense system of PBMC.

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How to Cite

Chanudom, L., & Tangpong, J. (2017). Scavenging Activities and Protective Effects of Syzygium Cumini (L.) Skeels on H2O2 Induce Oxidative Stress in Normal Human Peripheral Blood Mononuclear Cells. Journal of Health Research, 29(5), 315–322. Retrieved from https://he01.tci-thaijo.org/index.php/jhealthres/article/view/97193

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Section

ORIGINAL RESEARCH ARTICLE