Scavenging Activities and Protective Effects of Syzygium Cumini (L.) Skeels on H2O2 Induce Oxidative Stress in Normal Human Peripheral Blood Mononuclear Cells
Keywords:
Protective effects, Scavenging capacities, Syzygium cumini (L.) SkeelsAbstract
Syzygium cumini (L.) Skeels is the most commonly used as medicinal plants on diabetes and anti-inflammation. Hence, effects of aqueous leaf extracts of Syzygium cumini (L.) Skeels (ASC) on scavenging activities and protective effects of H2O2- induced oxidative damage in human peripheral blood mononuclear cells (PBMC) were determined in this study. Total phenolic and flavonoid contents of ASC at 2,000 µg/ml were 694.44±3.64 µgGAE/ml and 8.27±0.10 µg quercetin/ml respectively which were correlated with the r2 value at 0.93. IC50 values of ASC on free radical, hydroxyl and superoxide were 87.79±0.85, 766.62±28.61 and 1,299±25.13 µg/ml respectively while the nitric oxide scavenging activities were not higher than 50% at the concentration of 2,000 µg/ml of ASC. The protective effects of ASC in H2O2-induced PBMC cell damages were significantly increased to 83.13±3.57–112.32±4.47 % when treated with 0.25–1 µg/ml of ASC after oxidative induced by 5 mM of H2O2 for 3 h. Suggest that ASC is a potential source of natural antioxidant properties and protective defense system of PBMC.