Pharmacognostic Study Of Hydnocarpus Anthelminticus Seeds Endemic to Thailand

Abstract

The pharmacognostic specifications of Hydnocarpus anthelminticus Pierre ex Laness. (Flacourtiaceae) seeds were performed on 15 samples from 6 different geographical regions throughout Thailand.  The evaluations were performed according to the World Health Organization (WHO) guidelines for herbal standardization.  Microscopic investigation revealed numbers of aleurone grains, sclereid of seed coat and oil droplets.  The mean contents of foreign matter, total ash, acid insoluble ash, moisture, ethanol-soluble extractive, water-soluble extractive and loss on drying were 0.04, 1.61, 0.29, 8.83, 23.93, 3.95 and 7.38 %, respectively. TLC fingerprints were done by using the suitable mobile phase systems which were chloroform : methanol : acetic acid : water (50 : 30 : 8 : 3) or heptane : diethyl ether : acetic acid (70 : 30 : 2). This study is the first reported on phamacognostic study of H. anthelminticus Pierre ex Laness. seeds cultivated in Thailand.