In Vitro Free Radical Scavenging and Anti-Genotoxic Activities of Thunbergia Laurifolia Aqueous Leaf Extract

  • Mohammad Nasiruddin Rana School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand
  • Jitbanjong Tangpong School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand
Keywords: Thunbergia laurifolia, Genotoxicity, Lead, Lead poisoning, Antioxidant

Abstract

Background: Lead (Pb) is a xenobiotic, which causes numerous health defects like neurological, metabolic, kidney disorders, as well as mutation including DNA damage. Thunbergia laurifolia (TL) is a renowned medicinal plant for its antidote properties in Thailand. These properties caused us to investigate its anti-genotoxic effects against Pb in human peripheral blood leukocytes.

Methods: The TL aqueous leaf extract was prepared and the 50% inhibition concentration (IC50) on DPPH, superoxide, nitric oxide and hydroxyl radical scavenging activity determined. An in vitro, genotoxicity test was used to determine the magnitude of DNA damage of human peripheral blood leukocytes classified by a comet assay. TL extract at doses of 25, 50 and 100 µg/mL were used in combination with Pb at doses of 40 and 80 µg/mL, respectively, on in vitro human peripheral blood leukocytes and incubated at 37oC for 1 hrs. H2O2 (40 mM) was used as a DNA damage positive control.

Results: Genotoxicity was induced by Pb and H2O2 exposure in human peripheral blood leukocytes leading to DNA damage. TL was found to be a potent free radical scavenger of superoxide, hydroxyl radical, DPPH, hydrogen peroxide and nitric oxide with IC50 value of 0.298±0.004, 0.402±0.001, 0.869±0.030, 1.600±0.089 and 1.624±0.140 µg/mL, respectively. TL treatment ameliorated Pb induced genotoxicity in human peripheral blood leukocytes. Moreover, TL provided a protective effect on DNA damage against H2O2 in human peripheral blood leukocytes.

Conclusion: TL aqueous leaf extract possesses an anti-genotoxic effect, possibly due to its antioxidant capacity, and could prove useful as an antidote to lead toxicity.

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How to Cite
Rana, M. N., & Tangpong, J. (1). In Vitro Free Radical Scavenging and Anti-Genotoxic Activities of Thunbergia Laurifolia Aqueous Leaf Extract. Journal of Health Research, 31(2), 127-133. Retrieved from https://he01.tci-thaijo.org/index.php/jhealthres/article/view/79745
Section
ORIGINAL RESEARCH ARTICLE