In vitro study of 31P nuclear magnetic resonance in human red blood cells

Authors

  • Sompongse Chinayon Department of Medical Technology, Faculty of Medicine, Chulalongkorn University
  • Wanida Jinsart The Scientific and Technological Research Equipment Center, Chulalongkorn University
  • Somchai Eiam-ong Department of Medicine, Faculty of Medicine, Chulalongkorn University
  • Rampai Suvanpha Department of Medicine, Faculty of Medicine, Chulalongkorn University
  • Pongsak Pansin Department of Medicine, Faculty of Medicine, Chulalongkorn University

Keywords:

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Abstract

Phosphorus metabolites in human red blood cells were studied by a nuclear magnetic resonance spectrometer, JEOL FX 90Q, operating at 36.2 MHz. 31P  resonance signals of 2, 3- diphosPhoglycerate (DPG) and inorganic orthophosphate (Pi) were recorded in hemolysates and intact erythrocytes. However, the Pi peak was absent in the intact red blood cells, detecting for 4 hours (2,500 pulses). The chemical shift values (in ppm) of these biological phosphate compounds appearing in the spectrum were pH dependent.

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Published

2019-05-27

How to Cite

Chinayon, S., Jinsart, W., Eiam-ong, S., Suvanpha, R., & Pansin, P. (2019). In vitro study of 31P nuclear magnetic resonance in human red blood cells. Journal of Health Research, 2(1), 1–8. Retrieved from https://he01.tci-thaijo.org/index.php/jhealthres/article/view/191187

Issue

Vol. 2 No. 1: March 1988

Section

ORIGINAL RESEARCH ARTICLE