Development of Guava Liposome Serum and Evaluation of Free Radical-Scavenging Capacity

Abstract

The aims of the study were to formulate serum containing liposome with guava leaf (Psidium guajava Linn.) extract as for cosmetic. Free radical scavenging and antityrosinase property in term of IC50 of the extract from guava leaf was used as an outcome of the anti-wrinkle and whitening ability. The first part of this study was to evaluate the percentage of free radical inhibition and antityrosinase activity of the extract from guava leaf by DPPH method and dopachrome method respectively. The second part of this study was formulation of the serum containing liposome with guava leaf extract and evaluated free radical-scavenging capacity of the formulation compare to the crude extract by analyzing the phenolic compound in term of gallic acid. According to our study, the free radical scavenging property of the guava leaf extract was found effectively (IC50 = 53.1889 μg/ml) with antityrosinase activity calculated in the same term (IC50 = 9.864 mg/ml). The quantity of gallic acid were found in the extract and serum 3.0743 μg/mg of extract and 1.041 μg/ml respectively and percent of recovery were 67.73.