https://he01.tci-thaijo.org/index.php/ams <p>The <em><strong>Arch AHS</strong></em><em> </em>publishes various types of articles, including editorials, review article, original article, and letter to the editor. The journal’s scope encompasses entire spectrum of Allied Health Sciences ranging from basic to translational research, issuing 3 times a year.</p> Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. en-US Archives of Allied Health Sciences 2730-2008 https://he01.tci-thaijo.org/index.php/ams/article/view/269027 <p>Type 2 diabetes mellitus (T2DM) poses a global public health concern due to complications arising from insufficient knowledge of self-care practices. Despite the significant impact on blood glucose control and the prevention of foot amputation, research on the knowledge of exercise and foot care among individuals with uncontrolled T2DM in northeastern Thailand is lacking. Accurate comprehension of foot care and exercise knowledge is essential for these individuals to avoid inefficacy and potential harm. The objective of this study was to investigate knowledge toward exercise and foot care among individuals with uncontrolled T2DM at secondary care setting in northeastern Thailand. Data were gathered through a self-developed questionnaire with high internal consistency (KR-20 = 0.87) distributed to 170 uncontrolled T2DM patients residing in the Warichaphum district, Sakon Nakhon province. Descriptive statistics were employed for data analysis. Among these respondents, 58.82% demonstrated a ‘Good’ level of knowledge regarding exercise, while 85.71% showed a ‘Good’ level of knowledge regarding foot care. Nonetheless, respondents provided incorrect answers to several key items, highlighting the need to correct misconceptions for better comprehension. This study offers valuable insights for designing and implementing initiatives aimed at enhancing knowledge and promoting better exercise habits for blood sugar control among uncontrolled T2DM patients.</p> Ponlapat Yonglitthipagon Sakkarin Ponwiang Suriyan Thawinphai May Thandar Khin Patcharin Kumsong Copyright (c) 2024 Archives of Allied Health Sciences https://creativecommons.org/licenses/by-nc-nd/4.0 2024-05-28 2024-05-28 36 2 1 13 https://he01.tci-thaijo.org/index.php/ams/article/view/268147 <p>Mutation analysis of KRAS is necessary before starting treatment with monoclonal anti-EGFR antibodies for effective and appropriate treatment for individual patients. The objective of this study is to develop a Multiplex Allele-Specific Real-time PCR assay for analysis of the mutational status of KRAS codons 12 and 13, including 7 types of KRAS mutations (G12D, G12A, G12R, G12C, G12S, G12V, and G13D). 160 of FFPE colorectal cancer tissues were collected from Department of Medical Services, Institute of Pathology. DNA was isolated from the FFPE tissue using AS-primers specific for mutant DNA. Moreover, the MAS-Real-time PCR analysis of samples showed good concordance (K=0.837, 95% CI 0.740-0.933) with pyrosequencing. In addition, the MAS-Real-time assay has a sensitivity of 78.26% and specificity of 100%. Our developed MAS-Real-time PCR can be applied for detection of KRAS gene mutations in FFPE tissues which is a reliable, rapid, cost-effective method and not requiring advanced instruments.</p> Sirirat Seekhuntod Copyright (c) 2024 Archives of Allied Health Sciences https://creativecommons.org/licenses/by-nc-nd/4.0 2024-06-07 2024-06-07 36 2 14 29