Comparative study of chemical constituents and antioxidant activity of Benchalokawichian remedy in difference mixer water
Keywords:
Gas Chromatography-Mass Spectrometry, Benchalokawichian remedy, Difference mixer water, Harak remedy, AntioxidantAbstract
Benchalokawichian remedy is one of the Thai traditional medicines listed in the list of Herbal Medicinal Products of Thailand for antipyretic. Difference mixer water helps the drug have a flavorful taste, pleasant color, and good smell, to work faster and better, or enhance the effect of that herbal medicine formula.This study aimed to compare the chemical constituents of the Benchalokawichian remedy in 4 different mixer water: boiled water, jasmine water, ginger water, and coriander root water, which were also analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The antioxidant activity was analyzed via DPPH radical scavenging assay and the total phenolic content was analyzed by Folin-ciocalteau' reagent.
The result found that the Benchalokawichian remedy in boiled water, coriander root water, ginger water, and jasmine water contained about 16, 14, 17, and 19 different compounds, respectively. The Benchalokawichian remedy in jasmine water and coriander root water showed significantly higher antioxidants than the Benchalokawichian remedy in water mixer with IC50 values of 37.62 ± 2.08 and 42.50 ± 5.68 μg/ml, respectively. The results of this study according to the theory of Thai traditional medicine that has been used in conjunction with different mixer water to help increase the effectiveness of the drug. In conclusion, these results can be used as a development of the Benchalokawichian formula to be easy for intake and more effective.
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